TGFA anticorps (C-Term)

N° du produit ABIN6938541

Détail du produit anti-TGFA anticorps

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Épitope: C-Term

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Specificité: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Réactivité croisée (Details): Human. Cow. Rat. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)

Clone: MF9

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGFA (TGFA Produits)

Synonymes: anticorps TFGA, anticorps LOC100009482, anticorps TGF alpha, anticorps TGFA, anticorps wa-1, anticorps wa1, anticorps RATTGFAA, anticorps TGFAA, anticorps NV15606, anticorps si:ch211-10e8.7, anticorps transforming growth factor alpha, anticorps transforming growth factor alpha L homeolog, anticorps transforming growth factor-alpha, anticorps transforming growth factor, alpha, anticorps TGFA, anticorps TGF-A, anticorps Tgfa, anticorps tgfa.L, anticorps LOC100121300, anticorps LOC100009150, anticorps tgfa

Sujet: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1,TGF-alpha (Transforming Growth Factor alpha)
Cellular localisation: Cytoplasmic and Extracellular (secreted)

Poids moléculaire: ~6kDa

ID gène: 7039, 170009

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway