TGFA anticorps (AA 34-43)

N° du produit ABIN6938543

Cet anticorps Souris Monoclonal détecte spécifiquement TGFA dans IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Lapin et Poisson zèbre (Danio rerio).

Aperçu rapide pour TGFA anticorps (AA 34-43) (ABIN6938543)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain, Lapin, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TG86

Détail du produit anti-TGFA anticorps

Épitope: AA 34-43

Fonction: TGF-alpha (Transforming Growth Factor alpha) Antibody

Specificité: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Réactivité croisée (Details): Human. Rabbit. Zebrafish.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGFA

Sujet:

EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Jurkat or Ramos cells. Testicular Carcinoma.

Poids moléculaire: 6kDa

ID gène: 7039, 170009

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway