Topoisomerase II alpha anticorps (AA 1352-1493)

N° du produit ABIN6938635

Cet anticorps anti-Topoisomerase II alpha Monoclonal Souris (Clone TOP2A-1361) (ABIN6938635) détecte spécifiquement Topoisomerase II alpha dans WB, IHC et IF. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Topoisomerase II alpha anticorps (AA 1352-1493) (ABIN6938635)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: TOP2A-1361

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: AA 1352-1493

Fonction: Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Specificité: It recognizes a 170 kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Réactivité croisée (Details): Human. Mouse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A

HeLa cells. Tonsil.

Poids moléculaire: 170kDa

ID gène: 7153, 156346

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases