OLIG2 anticorps (AA 1-141)

N° du produit ABIN6939031

L’anticorps Souris Monoclonal anti-OLIG2 a été validé pour IHC (f). Il convient pour détecter OLIG2 dans des échantillons de Humain.

Aperçu rapide pour OLIG2 anticorps (AA 1-141) (ABIN6939031)

Antigène: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp OLIG2 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: OLIG2-2400

Détail du produit anti-OLIG2 anticorps

Épitope: AA 1-141

Specificité: Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).

Réactivité croisée (Details): Human,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human OLIG2 protein (around aa 1-141) (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application:

Positive Control: THP-1 cells. Astrocytoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur OLIG2

Antigène: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Autre désignation: OLIG2

Sujet: Basic helix loop helix protein class B1 (bHLHB1), basic helix-loop-helix protein 19 (bHLHe19), OLIG2, Oligodendrocyte lineage transcription factor 2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2 (PRKCBP2), RACK17,OLIG2 (Marker of Glial Brain Tumors)
Cellular localisation: Nuclear

Poids moléculaire: 30-40kDa

ID gène: 10215, 176977

UniProt: Q13516