HSPG2 anticorps

N° du produit ABIN6939672

L’anticorps Rat Monoclonal anti-HSPG2 a été validé pour IHC, IF, FACS, ISt et StM. Il convient pour détecter HSPG2 dans des échantillons de Humain, Souris, Boeuf (Vache), Poisson, Singe et Porc.

Aperçu rapide pour HSPG2 anticorps (ABIN6939672)

Antigène: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivité: Humain, Souris, Boeuf (Vache), Poisson, Singe, Porc

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPG2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Clone: A7L6

Détail du produit anti-HSPG2 anticorps

Specificité: This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Purification: Purified by Protein A/G

Immunogène: Murine EHS laminin preparation

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: Breast carcinomas, squamous cell carcinomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur HSPG2

Antigène: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Autre désignation: HSPG2

Poids moléculaire: >400kDa

ID gène: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism