FAS anticorps

N° du produit ABIN6939780

Cet anticorps anti-FAS Monoclonal Souris (Clone B-R18) (ABIN6939780) détecte spécifiquement FAS dans IHC, FACS, IF et Func. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour FAS anticorps (ABIN6939780)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: B-R18

Détail du produit anti-FAS anticorps

Fonction: CD95 / FAS / TNFRSF6 Antibody

Specificité: MAb B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human CD95 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).

Known Application: Functional Studies (Order Ab without Azide), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Frozen) (0.5-1 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: FAS

Sujet:

ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas, , Fas AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).

Poids moléculaire: 38-50kDa

ID gène: 355, 244139

UniProt: P25445

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity