MITF anticorps

N° du produit ABIN6940047

Cet anticorps Souris Monoclonal détecte spécifiquement MITF dans IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour MITF anticorps (ABIN6940047)

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MITF est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: D5

Détail du produit anti-MITF anticorps

Specificité: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: NH2 terminus fragment of human Mi protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MITF

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Autre désignation: MITF

Poids moléculaire: 52-56kDa (doublet)

ID gène: 4286

UniProt: O75030

Pathways: Chromatin Binding