Prohibitin anticorps (AA 167-261)

N° du produit ABIN6940303

Cet anticorps anti-Prohibitin est un anticorps Souris Monoclonal détectant Prohibitin dans WB, IHC, ELISA, StM et Coat. Adapté pour Humain.

Aperçu rapide pour Prohibitin anticorps (AA 167-261) (ABIN6940303)

Antigène: Prohibitin (PHB)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prohibitin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Staining Methods (StM), Coating (Coat)

Clone: SPM311

Détail du produit anti-Prohibitin anticorps

Épitope: AA 167-261

Specificité: Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Purification: Purified by Protein A/G

Immunogène: Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)

Isotype: IgG2c kappa

Information d'application

Indications d'application:

Positive Control: HepG2, A431 or MCF-7 cells. Liver, Kidney, Heart or Endometrium.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Prohibitin

Antigène: Prohibitin (PHB)

Autre désignation: PHB

Poids moléculaire: 30kDa

ID gène: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling