Cytochrome C anticorps

N° du produit ABIN6940318

Cet anticorps anti-Cytochrome C est un anticorps Souris Monoclonal détectant Cytochrome C dans FACS et IF. Adapté pour Humain, Rat et Souris.

Aperçu rapide pour Cytochrome C anticorps (ABIN6940318)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6H2-B4

Détail du produit anti-Cytochrome C anticorps

Purification: Purified by Protein A/G

Immunogène: Rat full-length cytochrome c protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: CYCS

Sujet: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Poids moléculaire: 15kDa

ID gène: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity