beta-2 Microglobulin anticorps

N° du produit ABIN6940415

Cet anticorps anti-beta-2 Microglobulin Monoclonal Souris (Clone B2M-961) (ABIN6940415) détecte spécifiquement beta-2 Microglobulin dans WB, IHC, FACS et IF. L’anticorps est réactif avec des échantillons de Humain et Non-Human Primate.

Aperçu rapide pour beta-2 Microglobulin anticorps (ABIN6940415)

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta-2 Microglobulin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B2M-961

Détail du produit anti-beta-2 Microglobulin anticorps

Fonction: Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody

Specificité: Recognizes a protein of 12 kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Full length recombinant human B2M protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Positive Control: HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-4 μg/mL), ,Western Blot (0.5-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur beta-2 Microglobulin

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Autre désignation: B2M

Sujet:

B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p

Cervix, Endometrial, HL-60 or HeLa cells. Carcinoma of Stomach, Kidney or Colon., Raji, THP-1

Poids moléculaire: 12kDa

ID gène: 567, 534255

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process