Cyclin D1 anticorps

N° du produit ABIN6940486

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin D1 dans WB, IHC, FACS, IF et StM. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour Cyclin D1 anticorps (ABIN6940486)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: DCS-6

Détail du produit anti-Cyclin D1 anticorps

Specificité: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40 % of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Purification: Purified by Protein A/G

Immunogène: Human full length recombinant cyclin D1 protein

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: CCND1

Poids moléculaire: 36kDa

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling