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Melanoma gp100 anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement Melanoma gp100 dans IHC, IF, FACS et StM. Il présente une réactivité envers Humain et Cheval.
N° du produit ABIN6940586

Aperçu rapide pour Melanoma gp100 anticorps (ABIN6940586)

Antigène

Voir toutes Melanoma gp100 (PMEL) Anticorps
Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité

  • 112
  • 12
  • 8
  • 7
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Humain, Cheval

Hôte

  • 61
  • 49
  • 2
  • 1
Souris

Clonalité

  • 62
  • 49
  • 2
Monoclonal

Conjugué

  • 55
  • 9
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Melanoma gp100 est non-conjugé

Application

  • 60
  • 60
  • 42
  • 41
  • 26
  • 20
  • 14
  • 7
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone

SPM286
  • Specificité

    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

    Aucune reactivité croisée

    Rat (Rattus)

    Purification

    Purified by Protein A/G

    Immunogène

    Membranes from a human melanoma metastasis

    Isotype

    IgG2b kappa
  • Indications d'application

    Positive Control: SK-MEL-28 cells. Melanoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Date de péremption

    24 months
  • Antigène

    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

    Autre désignation

    SILV

    Poids moléculaire

    90-100kDa

    ID gène

    6490

    UniProt

    P40967
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