TGFA anticorps (C-Term)

N° du produit ABIN6940715

Cet anticorps Souris Monoclonal détecte spécifiquement TGFA dans IHC, IF, ELISA, StM et Coat. Il présente une réactivité envers Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour TGFA anticorps (C-Term) (ABIN6940715)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Staining Methods (StM), Coating (Coat)

Clone: MF9

Détail du produit anti-TGFA anticorps

Épitope: C-Term

Specificité: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Purification: Purified by Protein A/G

Immunogène: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Jurkat or Ramos cells. Testicular Carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGFA

Poids moléculaire: ~6kDa

ID gène: 7039

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway