GRP94 anticorps

N° du produit ABIN6940802

Cet anticorps anti-GRP94 est un anticorps Rat Monoclonal détectant GRP94 dans WB, IHC, FACS, IF et StM. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval et Xenopus laevis.

Aperçu rapide pour GRP94 anticorps (ABIN6940802)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: 9G10-F8-2

Détail du produit anti-GRP94 anticorps

Specificité: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Purification: Purified by Protein A/G

Immunogène: Purified glucose regulated protein 94 (grp94) from chicken oviducts

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: HepG2, U20S cells. About 50 % of breast tumors express grp94 protein.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: HSP90B1

Poids moléculaire: 94kDa

ID gène: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades