Recombinant Ubiquitin B anticorps (AA 1-119)

N° du produit ABIN6940846

L’anticorps Lapin Monoclonal anti-Ubiquitin B a été validé pour IHC et StM. Il convient pour détecter Ubiquitin B dans des échantillons de Humain.

Aperçu rapide pour Recombinant Ubiquitin B anticorps (AA 1-119) (ABIN6940846)

Antigène: Ubiquitin B (UBB)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Ubiquitin B est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: UBB-3143R

Détail du produit anti-Ubiquitin B anticorps

Épitope: AA 1-119

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: HeLa or Jurkat cells. Alzheimer's Brain.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Ubiquitin B

Antigène: Ubiquitin B (UBB)

Autre désignation: UBB

Sujet: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Poids moléculaire: 9kDa

ID gène: 7314

UniProt: P62979, P62987, P62988

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway