Cyclin B2 anticorps

N° du produit ABIN6941010

L’anticorps Souris Monoclonal anti-Cyclin B2 a été validé pour ELISA, IHC et Coat. Il convient pour détecter Cyclin B2 dans des échantillons de Humain, Souris, Rat et Xenopus laevis.

Aperçu rapide pour Cyclin B2 anticorps (ABIN6941010)

Antigène: Cyclin B2 (CCNB2)

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B2 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Clone: X29-2

Détail du produit anti-Cyclin B2 anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin B2

Antigène: Cyclin B2 (CCNB2)

Autre désignation: CCNB2

Sujet: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Poids moléculaire: 51kDa

ID gène: 9133

UniProt: O95067

Pathways: Cycle Cellulaire, M Phase