Cyclin B2 anticorps

N° du produit ABIN6941010

L’anticorps anti-Cyclin B2 Monoclonal Souris est utilisé pour la détection de Cyclin B2 dans des échantillons de Humain, Souris, Rat et Xenopus laevis. Il a été validé pour ELISA.

Aperçu rapide pour Cyclin B2 anticorps (ABIN6941010)

Antigène: Cyclin B2 (CCNB2)

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B2 est non-conjugé

Application: ELISA

Clone: X29-2

Détail du produit anti-Cyclin B2 anticorps

Fonction: Cyclin B2 Antibody

Specificité: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin B2

Antigène: Cyclin B2 (CCNB2)

Autre désignation: CCNB2

Sujet:

ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694

HeLa cells. Human cervical tissue (IHC).

Poids moléculaire: 51kDa

ID gène: 9133, 194698

UniProt: O95067

Pathways: Cycle Cellulaire, M Phase