Recombinant Aurora Kinase B anticorps (AA 89-251)

N° du produit ABIN6941048

Cet anticorps anti-Aurora Kinase B est un anticorps Souris Monoclonal détectant Aurora Kinase B dans IHC et StM. Adapté pour Humain.

Aperçu rapide pour Recombinant Aurora Kinase B anticorps (AA 89-251) (ABIN6941048)

Antigène: Aurora Kinase B (AURKB)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Aurora Kinase B est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RAURKB-1592

Détail du produit anti-Aurora Kinase B anticorps

Épitope: AA 89-251

Specificité: Recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin s Lymphoma or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Aurora Kinase B

Antigène: Aurora Kinase B (AURKB)

Autre désignation: AURKB

Poids moléculaire: 39kDa

ID gène: 9212

UniProt: Q96GD4

Pathways: TCR Signaling, Cycle Cellulaire, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades