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CD14 anticorps (AA 25-148)

CD14 Reactivité: Humain WB, IHC, ISt, StM Hôte: Souris Monoclonal LPSR-2397 unconjugated
N° du produit ABIN6941095
  • Antigène Voir toutes CD14 Anticorps
    CD14
    Épitope
    • 16
    • 8
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 25-148
    Reactivité
    • 268
    • 55
    • 32
    • 30
    • 29
    • 28
    • 25
    • 24
    • 24
    • 18
    • 17
    • 14
    • 10
    • 9
    • 8
    • 8
    • 8
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 224
    • 64
    • 25
    • 3
    • 1
    Souris
    Clonalité
    • 253
    • 64
    Monoclonal
    Conjugué
    • 128
    • 30
    • 24
    • 18
    • 15
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD14 est non-conjugé
    Application
    • 224
    • 103
    • 64
    • 59
    • 53
    • 50
    • 22
    • 22
    • 21
    • 14
    • 14
    • 13
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
    Specificité
    Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)
    Clone
    LPSR-2397
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product CD14 Anticorps primaire
  • Indications d'application

    Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.

    Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    CD14
    Autre désignation
    CD14 (CD14 Produits)
    Synonymes
    anticorps CD14 molecule, anticorps CD14 antigen, anticorps CD14, anticorps Cd14
    Poids moléculaire
    55kDa
    ID gène
    929
    UniProt
    P08571
    Pathways
    Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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