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CD34 anticorps (CD34) Primary Antibody

CD34 Reactivité: Humain, Rat FACS, IF, IHC, StM, WB Hôte: Souris Monoclonal HPCA1-1171
N° du produit ABIN6941172
$508.20
Plus shipping costs $45.00
100 μg ABIN6941172
100 μg ABIN6941172
local_shipping Destination: Etats-Unis
Envoi sous 2 à 4 jours ouvrables
  • Antigène
    Reactivité
    Humain, Rat
    Hôte
    Souris
    Clonalité
    Monoclonal
    Conjugué
    Cet anticorp CD34 est non-conjugé
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
    Specificité
    This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant full-length human HPCA1 protein
    Clone
    HPCA1-1171
    Isotype
    IgG1, kappa
  • Indications d'application

    Positive Control: KG-1 cells. Human tonsil or angiosarcoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Autre désignation
    CD34 (CD34 Antibody Extrait)
    Poids moléculaire
    90-110kDa
    ID gène
    947
    UniProt
    P28906
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