CD63 anticorps (AA 100-197)

N° du produit ABIN6941226

Cet anticorps Souris Monoclonal détecte spécifiquement CD63 dans WB et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD63 anticorps (AA 100-197) (ABIN6941226)

Antigène: CD63

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: LAMP3-2790

Détail du produit anti-CD63 anticorps

Épitope: AA 100-197

Fonction: CD63 (Late Endosomes Marker) Antibody

Specificité: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndromeand is strongly expressed during the early stages of melanoma progression.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa 100-197) of human CD63 protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Spleen, melanoma or lymphoma tissues (IHC).

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD63

Antigène: CD63

Autre désignation: CD63

Sujet:

gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30

HL60, melanoma or lymphoma tissues (IHC)., SK-MEL-28, THP-1 or NIH/3T3 cells. Spleen

Poids moléculaire: 26kDa (core protein), 30-60kDa (glycosylated)

ID gène: 967, 445570

UniProt: P08962