CD79a anticorps (AA 202-216)

N° du produit ABIN6941266

Cet anticorps Souris Monoclonal détecte spécifiquement CD79a dans FACS, IF, WB, IHC et StM. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Porc et Singe.

Aperçu rapide pour CD79a anticorps (AA 202-216) (ABIN6941266)

Antigène: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD79a est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: JCB117-HM47-A9

Détail du produit anti-CD79a anticorps

Épitope: AA 202-216

Purification: Purified by Protein A/G

Immunogène: A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein (JCB117 and HM47/A9).

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Raji, Daudi or Ramos cells. Germinal center B- cells in a lymph node or tonsil.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD79a

Antigène: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Autre désignation: CD79A

Sujet: A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Poids moléculaire: 44kDa

ID gène: 973

UniProt: P11912

Pathways: BCR Signaling