KRT8, KRT18 anticorps

N° du produit ABIN6941355

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans IHC, StM, FACS, IF et WB. Adapté pour Humain, Boeuf (Vache), Singe, Porc et Mouton.

Aperçu rapide pour KRT8, KRT18 anticorps (ABIN6941355)

Antigène: KRT8, KRT18

Reactivité: Humain, Boeuf (Vache), Singe, Porc, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: C-51

Détail du produit

Specificité: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Aucune reactivité croisée: Poulet, Âne, Furet, Souris, Lapin, Rat (Rattus), Xenopus laevis

Purification: Purified by Protein A/G

Immunogène: Cytoskeleton preparation from HeLa cells

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: KRT8, KRT18

Autre désignation: KRT8 & KRT18

Poids moléculaire: 52.5kDa (CK8), 45kDa (CK18)

ID gène: 3856, 3875

UniProt: P05787, P05783