ATF6 anticorps (AA 301-400) (Cy5)

N° du produit ABIN6942166

L’anticorps Lapin Polyclonal anti-ATF6 a été validé pour WB, IF (p) et IF (cc). Il convient pour détecter ATF6 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ATF6 anticorps (AA 301-400) (Cy5) (ABIN6942166)

Antigène: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATF6 est conjugé à/à la Cy5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Détail du produit anti-ATF6 anticorps (Cy5)

Épitope: AA 301-400

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human ATF6

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur ATF6

Antigène: ATF6 (Activating Transcription Factor 6 (ATF6))

Autre désignation: ATF6

Sujet:

Synonyms: Activating transcription factor 6 alpha, Activating transcription factor 6, ATF 6, ATF6 alpha, ATF6, ATF6-alpha, ATF6A, ATF6A_HUMAN, cAMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Cyclic AMP dependent transcription factor ATF 6 alpha, DKFZp686P2194, ESTM49, FLJ21663, Processed cyclic AMP dependent transcription factor ATF 6 alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha.

Background: ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It also interacts with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site 1 and site 2 proteases. ATF6 is N glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.

ID gène: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response