Nibrin anticorps

N° du produit ABIN6944404

Détail du produit anti-Nibrin anticorps

Antigène: Nibrin (NBN)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Nibrin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Clone: 3G5

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur Nibrin

Antigène: Nibrin (NBN)

Autre désignation: p95 NBS1 (NBN Produits)

Synonymes: anticorps NBN, anticorps AT-V1, anticorps AT-V2, anticorps ATV, anticorps NBS, anticorps NBS1, anticorps P95, anticorps Nbs1, anticorps im:6911679, anticorps zgc:194152, anticorps nibrin, anticorps NBN, anticorps nbn, anticorps Nbn

Sujet:

Synonyms: Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, NBN

Background: Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.

ID gène: 4683

UniProt: O60934

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response