Recombinant PCNA anticorps (AA 100-200)

N° du produit ABIN6944453

Cet anticorps anti-PCNA Monoclonal Lapin (ABIN6944453) détecte spécifiquement PCNA dans WB, FACS, IF, IHC (p), ICC et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant PCNA anticorps (AA 100-200) (ABIN6944453)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-PCNA anticorps

Épitope: AA 100-200

Fonction: PCNA Recombinant Antibody

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: Recombinant human PCNA between 100 to 200 amino acids

Isotype: IgG

Information d'application

Indications d'application: WB(1:300-5000), IHC-P(1:20-100), IHC-F(1:200-400), IF(1:50-200), Flow-Cyt(1 μg/Test), ICC/IF(1:50-100)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet:

Synonyms: ProlifeRating cell nuclear antigen, Cyclin, PCNA

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy