Prohibitin anticorps

N° du produit ABIN6944678

Cet anticorps anti-Prohibitin est un anticorps Lapin Monoclonal détectant Prohibitin dans WB, FACS, IHC (p), IF (cc) et IHC (fro). Adapté pour Humain, Souris, Rat, Poisson zèbre (Danio rerio), Crucian Carp et Poisson.

Aperçu rapide pour Prohibitin anticorps (ABIN6944678)

Antigène: Prohibitin (PHB)

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Crucian Carp, Poisson

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Prohibitin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10

Détail du produit anti-Prohibitin anticorps

Réactivité croisée: Poisson, Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Réactivité croisée (Details): Crucian carp (Carassius carassius)

Purification: Purified by Protein A.

Immunogène: N terminal Human Prohibitin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur Prohibitin

Antigène: Prohibitin (PHB)

Autre désignation: Prohibitin

Sujet:

Synonyms: Prohibitin, PHB

Background: Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80 % of the cell lines analyzed.

ID gène: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling