Interferon gamma anticorps (PromoFluor 647 Premium)

N° du produit ABIN6963751

Cet anticorps anti-Interferon gamma est un anticorps Souris Monoclonal détectant Interferon gamma dans FACS. Adapté pour Lapin.

Aperçu rapide pour Interferon gamma anticorps (PromoFluor 647 Premium) (ABIN6963751)

Antigène: Interferon gamma (IFNG)

Reactivité: Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Interferon gamma est conjugé à/à la PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: MT318

Détail du produit anti-Interferon gamma anticorps (PromoFluor 647 Premium)

Specificité: Native and recombinant IFN-γ

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant rabbit IFN-gamma

Isotype: IgG2a

Information d'application

Indications d'application: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable rabbit spleen cells. Cells were stimulated for 5 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with CD8-PE and MT318-PF647P (right panel). The left panel depicts cells stained with a matched isotype control antibody. The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFNG

ID gène: 100008602

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy