Interferon gamma anticorps (PromoFluor 647 Premium)

N° du produit ABIN6963777

L’anticorps Souris Monoclonal anti-Interferon gamma a été validé pour FACS. Il convient pour détecter Interferon gamma dans des échantillons de Boeuf (Vache), Mouton et Chévre. Il y a 1 publication disponible.

Aperçu rapide pour Interferon gamma anticorps (PromoFluor 647 Premium) (ABIN6963777)

Antigène: Interferon gamma (IFNG)

Reactivité: Boeuf (Vache), Mouton, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Interferon gamma est conjugé à/à la PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: MT307

Détail du produit anti-Interferon gamma anticorps (PromoFluor 647 Premium)

Specificité: Native and recombinant IFN-γ.

Réactivité croisée (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant bovine IFN-gamma

Isotype: IgG2a

Information d'application

Indications d'application: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable bovine PBMC. Cells were stimulated for 16 hours in the presence of PHA and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT307-PF647P (left panel). The specificity of MT307-PF647P was demonstrated by pre-incubating the antibody with recombinant bovine IFN-γ prior to staining of PBMC (middle panel). Matched isotype control antibody (right panel). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Références pour anticorps anti-Interferon gamma (PromoFluor 647 Premium) (ABIN6963777)

Richt, Kasinathan, Hamir, Castilla, Sathiyaseelan, Vargas, Sathiyaseelan, Wu, Matsushita, Koster, Kato, Ishida, Soto, Robl, Kuroiwa: "Production of cattle lacking prion protein." dans: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFNG

ID gène: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy