Interferon gamma anticorps (PromoFluor-488 Premium)

N° du produit ABIN6963784

Cet anticorps anti-Interferon gamma est un anticorps Souris Monoclonal détectant Interferon gamma dans FACS. Adapté pour Cobaye.

Aperçu rapide pour Interferon gamma anticorps (PromoFluor-488 Premium) (ABIN6963784)

Antigène: Interferon gamma (IFNG)

Reactivité: Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Interferon gamma est conjugé à/à la PromoFluor-488 Premium

Application: Flow Cytometry (FACS)

Clone: MT4A27

Détail du produit anti-Interferon gamma anticorps (PromoFluor-488 Premium)

Specificité: Native and recombinant IFN-γ

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant guinea pig IFN-gamma

Isotype: IgG1

Information d'application

Indications d'application: Detection of guinea pig IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm. Detection of IFN-γ by flow cytometry in viable guinea pig spleen cells. Cells were stimulated for 16 hrs in the presence of PMA/ ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT4A27-PF488P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFNG

ID gène: 100379568

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy