H2AFX anticorps (pTyr142)

N° du produit ABIN6971768

Cet anticorps Lapin Polyclonal détecte spécifiquement H2AFX dans WB et DB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour H2AFX anticorps (pTyr142) (ABIN6971768)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-H2AFX anticorps

Épitope: pTyr142

Attributs du produit: Histone H2A.XY142ph (H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. Tyrosine phosphorylation at position 142 on Histone H2A.X is constitutive and is mediated by BAZ1B/WSTF. This mark distinguishes between apoptotic and DNA repair responses to genotoxic stress. Histone H2A.XY142ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogène: This antibody was raised against a peptide containing phospho-tyrosine 142 of human histone H2A.X.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.X

Poids moléculaire: 15 kDa

NCBI Accession: NP_002096

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus