PD-L1 anticorps (Isoform 1)

N° du produit ABIN6990335

L’anticorps Lapin Polyclonal anti-PD-L1 a été validé pour WB, FACS, ELISA, IF et IHC (p). Il convient pour détecter PD-L1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PD-L1 anticorps (Isoform 1) (ABIN6990335)

Antigène: PD-L1 (CD274 (PD-L1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PD-L1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PD-L1 anticorps

Épitope: AA 60-110, Isoform 1

Specificité: PD-L1 antibody has no cross-reactivity to PD-L2.

Purification: PD-L1 Antibody is affinity chromatography purified via peptide column.

Immunogène: Anti-PD-L1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human PD-L1 isoform 1. The immunogen is located within amino acids 60 - 110 of PD-L1.

Isotype: IgG

Information d'application

Indications d'application: WB: 1-2 μ,g/mL, IHC-P: 2.5-5 μ,g/mL, IF: 20 μ,g/mL, Flow Cyt: 0.5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human and rat samples, Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PD-L1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur PD-L1

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1

Sujet: PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).

Poids moléculaire: Predicted: 33 kDa

Observed: 37 kDa

ID gène: 29126

NCBI Accession: NP_054862

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints