TRIM5 anticorps (C-Term)

N° du produit ABIN6990446

L’anticorps anti-TRIM5 Polyclonal Lapin est utilisé pour la détection de TRIM5 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC (p) et IF.

Aperçu rapide pour TRIM5 anticorps (C-Term) (ABIN6990446)

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-TRIM5 anticorps

Épitope: C-Term

Purification: TRIM5 delta Antibody is affinity chromatography purified via peptide column.

Immunogène: TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta. The immunogen is located within the last 50 amino acids of TRIM5 delta.

Isotype: IgG

Information d'application

Indications d'application: TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μ,g/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM5 delta Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: TRIM5 delta antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur TRIM5

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Autre désignation: TRIM5 delta

Sujet: TRIM5 delta Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α, variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5δ, isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ, also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

Poids moléculaire: Predicted: 36 kDa

Observed: 33 kDa

ID gène: 85363

NCBI Accession: NP_149084

UniProt: Q9C035

Pathways: Activation of Innate immune Response