PUMA anticorps (C-Term, Isoform 1)

N° du produit ABIN6990494

L’anticorps Lapin Polyclonal anti-PUMA a été validé pour WB, ELISA, ICC et IF. Il convient pour détecter PUMA dans des échantillons de Humain et Souris.

Aperçu rapide pour PUMA anticorps (C-Term, Isoform 1) (ABIN6990494)

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUMA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-PUMA anticorps

Épitope: C-Term, Isoform 1

Specificité: A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.

Purification: PUMA Antibody is affinity chromatography purified via peptide column.

Immunogène: Anti-PUMA antibody was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus human PUMA isoform 1. The immunogen is located within the last 50 amino acids of PUMA.

Isotype: IgG

Information d'application

Indications d'application: WB: 1-4 μ,g/mL, IF: 2 μ,g/mL, ICC: 1 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PUMA Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: PUMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur PUMA

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Autre désignation: PUMA

Sujet: PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Poids moléculaire: 23 kDa

ID gène: 27113

NCBI Accession: NP_055232

UniProt: Q96PG8

Pathways: Signalisation p53, Positive Regulation of Endopeptidase Activity