LAMP1 anticorps (AA 230-280)

N° du produit ABIN6990580

Cet anticorps Lapin Polyclonal détecte spécifiquement LAMP1 dans WB, IF, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour LAMP1 anticorps (AA 230-280) (ABIN6990580)

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LAMP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LAMP1 anticorps

Épitope: AA 230-280

Specificité: LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE.

Purification: LAMP-1 Antibody is affinity chromatography purified via peptide column.

Immunogène: LAMP-1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human LAMP-1. The immunogen is located within amino acids 230 - 280 of LAMP-1.

Isotype: IgG

Information d'application

Indications d'application: LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: LAMP-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: LAMP-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: LAMP-1

Sujet: LAMP-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.

Poids moléculaire: Predicted: 46 kDa

Observed: 95 kDa

ID gène: 3916

NCBI Accession: NP_005552

UniProt: P11279

Pathways: Autophagy