ITCH anticorps (N-Term)

N° du produit ABIN6991954

Détail du produit anti-ITCH anticorps

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Épitope: AA 40-90, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ITCH est non-conjugé

Application: ELISA, Western Blotting (WB)

Specificité: ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist, this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.

Purification: ITCH antibody is affinity chromatography purified via peptide column.

Immunogène: ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH . The immunogen is located within amino acids 40 - 90 of ITCH.

Isotype: IgG

Information d'application

Indications d'application: ITCH antibody can be used for detection of ITCH by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: ITCH antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: ITCH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur ITCH

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH (ITCH Produits)

Synonymes: anticorps AIF4, anticorps AIP4, anticorps NAPP1, anticorps dJ468O1.1, anticorps 6720481N21Rik, anticorps 8030492O04Rik, anticorps A130065M08, anticorps C230047C07Rik, anticorps itchy E3 ubiquitin protein ligase, anticorps itchy, E3 ubiquitin protein ligase, anticorps ITCH, anticorps Itch

Sujet: The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).

Poids moléculaire: Predicted: 99 kDa

Observed: 99 kDa

ID gène: 83737

NCBI Accession: NP_001244066

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events