SCRIB anticorps (Internal Region)

N° du produit ABIN7013862

Cet anticorps Chèvre Polyclonal détecte spécifiquement SCRIB dans WB, ELISA et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour SCRIB anticorps (Internal Region) (ABIN7013862)

Antigène: SCRIB (Scribbled Homolog (SCRIB))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SCRIB est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-SCRIB anticorps

Épitope: Internal Region

Fonction: SCRIB / Scribble

Séquence: PEGPGKEKEL PGQ

Specificité: This antibody is expected to recognize both reported isoforms (NP_874365.3, NP_056171.3).

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Information d'application

Indications d'application:

Western Blot: Approx 200-250 kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178 kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary inc

Peptide ELISA: antibody detection limit dilution 1:1000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur SCRIB

Antigène: SCRIB (Scribbled Homolog (SCRIB))

Autre désignation: SCRIB

Sujet: SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble

ID gène: 23513

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization