EGLN1 anticorps (AA 42-140) (Biotin)

N° du produit ABIN702642

Cet anticorps anti-EGLN1 est un anticorps Lapin Polyclonal détectant EGLN1 dans WB, ELISA, IHC (p) et IHC (fro). Adapté pour Humain et Souris.

Aperçu rapide pour EGLN1 anticorps (AA 42-140) (Biotin) (ABIN702642)

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EGLN1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-EGLN1 anticorps (Biotin)

Épitope: AA 42-140

Réactivité croisée: Humain, Souris

Homologie: Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PHD2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur EGLN1

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Autre désignation: PHD2

Sujet:

Synonyms: HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2, Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, HIF-prolyl hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, SM-20, EGLN1, PNAS-118, PNAS-137

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferencially recognized via a LXXLAP motif.

ID gène: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, L'effet Warburg