CD73 anticorps (Extracellular, N-Term)

N° du produit ABIN7042877

L’anticorps anti-CD73 Polyclonal Lapin est utilisé pour la détection de CD73 dans des échantillons de Souris. Il a été validé pour WB, IHC, FACS, IF et LCI.

Aperçu rapide pour CD73 anticorps (Extracellular, N-Term) (ABIN7042877)

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD73 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Détail du produit anti-CD73 anticorps

Épitope: AA 429-433, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to CD73

Réactivité croisée: Humain, Souris, Rat

Homologie: Human,rat - identical

Attributs du produit: Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7234599)

Blocking Peptide: (ABIN7234599)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CD73

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: NT5E

Sujet:

Synonyms: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E

Description: CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

ID gène: 23959

NCBI Accession: NM_001204813

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process