ASIC3 anticorps (Intracellular)

N° du produit ABIN7042917

Cet anticorps anti-ASIC3 est un anticorps Lapin Polyclonal détectant ASIC3 dans WB, IHC, IF et IC. Adapté pour Rat.

Aperçu rapide pour ASIC3 anticorps (Intracellular) (ABIN7042917)

Antigène: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASIC3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-ASIC3 anticorps

Épitope: AA 2-16, Intracellular

Fonction: A Rabbit Polyclonal Antibody to ASIC3 Channel

Specificité: Intracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: 15 amino acid residues identical, human - 11,Mouse - 14

Attributs du produit: Anti-ASIC3 Antibody (ABIN7042917, ABIN7045243 and ABIN7045244) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Commentaires:

Cited Application: IHC

Negative Control: (ABIN7234708)

Blocking Peptide: (ABIN7234708)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur ASIC3

Antigène: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Autre désignation: ASIC3

Sujet: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC,ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Alternative names: ASIC3, Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

ID gène: 286920

NCBI Accession: NM_004769

UniProt: O35240