FZD1 anticorps (Extracellular)

N° du produit ABIN7043140

Cet anticorps Lapin Polyclonal détecte spécifiquement FZD1 dans WB, FACS et LCI. Il présente une réactivité envers Rat.

Aperçu rapide pour FZD1 anticorps (Extracellular) (ABIN7043140)

Antigène: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-FZD1 anticorps

Épitope: AA 238-250, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Frizzled-1

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Anti-Frizzled-1 (FZD1) (extracellular) Antibody (ABIN7043140, ABIN7044291 and ABIN7044292) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PEFWTSNPQHGGG, corresponding to amino acid residues 238-250 of rat Frizzled-1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235337)

Blocking Peptide: (ABIN7235337)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur FZD1

Antigène: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Autre désignation: FZD1

Sujet: Frizzled class receptor 1, Fz-1,Frizzled class receptor 1 (also known as FZD1) belongs to the frizzled subfamily of G protein-coupled receptors. Currently, the frizzled family of GPCRs contains 10 frizzled proteins (FZD1-10) and Smoothened (SMO).Similar to other GPCRs, FZD1 consists of a single polypeptide with an extracellular N-terminus, an intracellular C-terminus, seven hydrophobic transmembrane domains (TM1-TM7) linked by three extracellular loops (ECL1-ECL3) and three intracellular loops (ICL1-ICL3). FZD1 is encoded by the firzzled-1 gene which is mainly expressed in the adult heart, placenta, lung, kidney, pancreas, prostate and ovary.FZD1 is activated by WNTs, which are cysteine-rich lipo-glycoproteins with fundamental functions in ontogeny and tissue homeostasis. Signaling through the WNT/FZD complex can lead to the activation of pertussis toxin-sensitive heterotrimeric G proteins, the elevation of intracellular calcium activation of cGMP-specific PDE6 and elevation of cAMP as well as RAC-1, JNK, Rho and Rho kinase signaling1.The relation between the FZD1 pathway and various pathologies has been researched extensively. Reduced levels of FZD1 can be found in the spinal cords of ALS transgenic mice, possibly due to the increase of FZD1 protein degradation or the inhibition of FZD1 protein expression in the spinal cord in the disease process of ALS transgenic mice2. The FZD1 signaling pathway has also been implicated in inflammatory bowel disease. Patients with ulcerative colitis exhibit reduced expression of FZD1. FZD1 is overexpressed in the multi drug resistant cancer cell subline MCF-7/ADM and seems to mediate multidrug resistance by regulating the Wnt/β-catenin pathway3.

Alternative names: Frizzled-1 (FZD1), Frizzled class receptor 1, Fz-1

ID gène: 58868

NCBI Accession: NM_003505

UniProt: Q08463

Pathways: Signalisation WNT, Asymmetric Protein Localization