Metabotropic Glutamate Receptor 1 anticorps (Extracellular) (Atto 488)

N° du produit ABIN7043246

L’anticorps anti-Metabotropic Glutamate Receptor 1 Polyclonal Lapin est utilisé pour la détection de Metabotropic Glutamate Receptor 1 dans des échantillons de Rat. Il a été validé pour IHC et IF.

Aperçu rapide pour Metabotropic Glutamate Receptor 1 anticorps (Extracellular) (Atto 488) (ABIN7043246)

Antigène: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Metabotropic Glutamate Receptor 1 est conjugé à/à la Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Metabotropic Glutamate Receptor 1 anticorps (Atto 488)

Épitope: AA 501-516, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1 Conjugated to the Fluorescent Dye ATTO-488

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human,dog - identical

Attributs du produit: Anti-mGluR1 (extracellular) Antibody (ABIN7043245, ABIN7044330 and ABIN7044331) is a highly specific antibody directed against the extracellular N-terminus domain of rat mGluR1. The antibody can be used in western blot and immunocytochemistry applications, and recognizes mGluR1 in rat, mouse and human samples. \nAnti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody (ABIN7043246) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:20-1:60

Application Dilutions Western blot wb: N/A

Commentaires:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7235681)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur Metabotropic Glutamate Receptor 1

Antigène: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Autre désignation: GRM1

Sujet: Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13,L-Glutamate is the major excitatory neurotransmitter in the central nervous system. It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus and an intracellular C-terminus. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11, that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. Additional downstream signaling pathways include the activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins have also been reported.mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Alternative names: mGluR1, Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13

ID gène: 24414

NCBI Accession: NM_000838

UniProt: P23385