ChT anticorps (Extracellular)

N° du produit ABIN7043267

L’anticorps Lapin Polyclonal anti-ChT a été validé pour WB, IHC, IF, IC et LCI. Il convient pour détecter ChT dans des échantillons de Souris.

Aperçu rapide pour ChT anticorps (Extracellular) (ABIN7043267)

Antigène: ChT (High Affinity Choline Transporter (ChT))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ChT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Détail du produit anti-ChT anticorps

Épitope: AA 299-311, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)

Specificité: 4th extracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat,human - 12,13 amino acid residues identical

Attributs du produit: Anti-Choline Transporter (SLC5A7) (extracellular) Antibody is directed against an epitope of mouse CHT. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (ABIN7043267 and ABIN7044218) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CHT from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline transporter

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur ChT

Antigène: ChT (High Affinity Choline Transporter (ChT))

Autre désignation: SLC5A7

Sujet: Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1,In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released to the synaptic cleft via synaptic vesicles and activates muscarinic and nicotinic receptors located on postsynaptic membranes1,2. Acetylcholine is then rapidly hydrolyzed into choline and acetate. Since choline is not synthesized de novo and thereby only made available through diet, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT)1,2.CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus3,4. CHT belongs to the Na+-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM1. The activity of CHT reflects that of neurons1,5 i.e. increased CHT activity indicates an increase in neuronal activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-terminal1,2.Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons6.

Alternative names: Choline Transporter (SLC5A7), Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1

ID gène: 63993

NCBI Accession: NM_001305005

UniProt: Q8BGY9