KIDINS220 anticorps (Intracellular)

N° du produit ABIN7043293

Cet anticorps Lapin Polyclonal détecte spécifiquement KIDINS220 dans WB, IHC et IF. Il présente une réactivité envers Rat.

Aperçu rapide pour KIDINS220 anticorps (Intracellular) (ABIN7043293)

Antigène: KIDINS220 (Kinase D-Interacting Substrate, 220kDa (KIDINS220))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIDINS220 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-KIDINS220 anticorps

Épitope: AA 1335-1348, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Kidins220

Specificité: Intracellular, C-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Anti-Kidins220 Antibody (ABIN7043293, ABIN7045160 and ABIN7045161) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kidins220 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PRHSNLSWQSQTRR, corresponding to amino acid residues 1335-1348 of rat Kidins220

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Commentaires:

Cited Application: IHC

Negative Control: (ABIN7235829)

Blocking Peptide: (ABIN7235829)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur KIDINS220

Antigène: KIDINS220 (Kinase D-Interacting Substrate, 220kDa (KIDINS220))

Autre désignation: KIDINS220

Sujet: Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS,The Kidins220 (Kinase D-interacting substrate of 220 kDa) or ARMS (Ankyrin repeat-rich membrane-spanning) protein was originally identified as a substrate for protein kinase D (PKD) in neural cells. Kidins220 is a member of the Kidins220/ARMS and PifA (KAP) family of P-loop nucleotide phosphatases (NTPases).Kidins220 is an integral membrane protein, containing four transmembrane segments in the central part of the molecule, and N- and C-terminal tails both exposed to the cytoplasm. Kidins220 possesses numerous domains that mediate protein-protein interactions. The N-terminus of Kidins220 contains eleven ankyrin repeats which form a concave binding surface that is accessible to molecular partners. Kidins220 contains Walker A and Walker B (involved in the binding of nucleotides) motifs in its juxta-membrane regions flanking the N- and C-termini. The C-terminus of Kidins220 includes several protein-protein interaction domains that are involved in inter- and intra-molecular interactions. The last four amino acids of Kidins220 constitute a PDZ-binding motif that binds the PDZ domain of α-syntrophin, thereby forming a complex that is associated with the EphA4 receptor at the neuromuscular junction1.Neurotrophin signaling is responsible for a wide variety of neuronal functions and is dependent upon two different transmembrane receptor types, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor that belongs to the TNF receptor family. Kidins220 interacts with TrkA, TrkB, TrkC receptors, and with p75NTR. Kidinis220 is rapidly tyrosine phosphorylated after binding of neurotrophins to Trk receptors and provides a docking site for the CrkL-C3G complex, resulting in Rap1-dependent sustained ERK activation2.Kidins220 also associates with NMDA receptors. Upon overstimulation of the NMDA receptor, activity-dependent Ca2+ influx through NMDA receptors leads to a decrease in Kidins220 levels owing to transcriptional downregulation and protein cleavage by calpain1.

Alternative names: Kidins220, Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS

ID gène: 116478

NCBI Accession: NM_020738

UniProt: Q9EQG6