ORAI1 anticorps (Extracellular) (Biotin)

N° du produit ABIN7043410

Cet anticorps Souris Monoclonal détecte spécifiquement ORAI1 dans WB et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour ORAI1 anticorps (Extracellular) (Biotin) (ABIN7043410)

Antigène: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ORAI1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 3F11-D10-B9

Détail du produit anti-ORAI1 anticorps (Biotin)

Épitope: Extracellular

Fonction: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin

Specificité: Extracellular

Réactivité croisée (Details): Recognizes only human Orai1.

Homologie: Recognizes Orai1 from human samples only

Attributs du produit: Mouse Anti-Human Orai1 (extracellular) Antibody (ABIN7043409 and ABIN7044554) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only. \nMouse Anti-Human Orai1 (extracellular)-Biotin Antibody (ABIN7043409 and ABIN7044554)-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody has been tested in western blot and direct flow cytometry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: Synthetic mapping to an extracellular sequence of human Orai1

Isotype: IgM

Information d'application

Indications d'application:

Antigen preadsorption control: NA

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur ORAI1

Antigène: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Autre désignation: ORAI1

Sujet: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1,Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified, Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

ID gène: 84876

NCBI Accession: NM_032790

UniProt: Q63633

Pathways: TCR Signaling, BCR Signaling