P2RX7 anticorps (Extracellular)

N° du produit ABIN7043578

Cet anticorps Lapin Polyclonal détecte spécifiquement P2RX7 dans WB, IHC, IF, FACS, LCI et IC. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour P2RX7 anticorps (Extracellular) (ABIN7043578)

Antigène: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp P2RX7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Classe de qualité: KO Validated

Détail du produit anti-P2RX7 anticorps

Épitope: AA 136-152, Extracellular

Fonction: A Rabbit Polyclonal Antibody to P2X7 Receptor

Specificité: Extracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: Human,rat - identical, bovine - 14,17 amino acid residues identical

Attributs du produit: Anti-P2X7 Receptor (extracellular) Antibody (ABIN7043578, ABIN7045080 and ABIN7045081) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Commentaires:

Cited Application: IHC|ICC|IFC

Negative Control: (ABIN7236676)

Blocking Peptide: (ABIN7236676)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur P2RX7

Antigène: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Autre désignation: P2RX7

Sujet: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor,The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4

Alternative names: P2X7 Receptor, Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

ID gène: 18439

NCBI Accession: NM_002562

UniProt: Q9H244

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis