Serotonin Receptor 4 anticorps (Extracellular) (FITC)

N° du produit ABIN7043625

Cet anticorps anti-Serotonin Receptor 4 est un anticorps Lapin Polyclonal détectant Serotonin Receptor 4 dans FACS et LCI. Adapté pour Rat.

Aperçu rapide pour Serotonin Receptor 4 anticorps (Extracellular) (FITC) (ABIN7043625)

Antigène: Serotonin Receptor 4 (HTR4)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Serotonin Receptor 4 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-Serotonin Receptor 4 anticorps (FITC)

Épitope: AA 168-180, Extracellular

Fonction: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC

Specificité: 2nd extracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse - 12,13 amino acid residues identical, human - 11

Attributs du produit: Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (ABIN7043624, ABIN7045290 and ABIN7045291) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples. \nAnti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (ABIN7043624, ABIN7045290 and ABIN7045291)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Commentaires:

Negative Control: (ABIN7582044)

Blocking Peptide:

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur Serotonin Receptor 4

Antigène: Serotonin Receptor 4 (HTR4)

Autre désignation: HTR4

Sujet: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4,5-hydroxytryptamine receptor 4 (5-HT4 receptor) is a subclass among the large family of receptors mediating serotonin signaling at central and peripheral targets. 5-HT4 receptor activation plays a role in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.5-HT4 receptor is a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs) and couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is cytoplasmic3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Alternative names: 5HT4 Receptor (HTR4), 5-Hydroxytryptamine receptor 4, Serotonin receptor 4

ID gène: 25324

NCBI Accession: NM_000870

UniProt: Q62758

Pathways: Signalistation JAK/STAT, cAMP Metabolic Process, S100 Proteins