SNCA anticorps (C-Term, Intracellular)

N° du produit ABIN7043800

Cet anticorps Cobaye Polyclonal détecte spécifiquement SNCA dans WB et IHC. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour SNCA anticorps (C-Term, Intracellular) (ABIN7043800)

Antigène: SNCA (Synuclein, alpha (SNCA))

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp SNCA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SNCA anticorps

Épitope: AA 97-110, C-Term, Intracellular

Fonction: A Guinea Pig Polyclonal Antibody to α-Synuclein

Réactivité croisée: Souris, Rat

Homologie: Mouse - identical, human - 11 out of 14 amino acid residues identical

Attributs du produit: Guinea Pig Anti-Alpha-Synuclein Antibody is a highly specific antibody directed against an epitope of rat α-Synuclein (SNCA). The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize α-Synuclein from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Alpha-Synuclein Antibody (ABIN7043799, ABIN7045180 and ABIN7045181) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat alpha-synuclein

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7237358)

Blocking Peptide: (ABIN7237358)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur SNCA

Antigène: SNCA (Synuclein, alpha (SNCA))

Autre désignation: SNCA

Sujet:

Synonyms: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA

Description: α-Synuclein belongs to the Synuclein family of proteins containing three other members (β-synuclein, γ-synuclein and synoretnin). α-Synuclein is a highly conserved acidic protein that is concentrated mainly in presynaptic axon terminals.The gene encoding α-synuclein is known as SNCA or PARK1. α-Synuclein, together with other members of its family, lacks typical secondary structure. In its primary structure, α-synuclein is characterized by seven imperfect 11-residue repeat sequences that are predicted to form five amphipatic helices on the amino terminal half. The acidic, glutamate rich carboxy-terminal region remains unstructured even in the presence of membranes. α-Synuclein helices 1-4 are predicted to associate with lipid vesicles while helix 5 would be responsible for protein-to-protein interaction. The protein interacts and with DAT, the dopamine transporter, and thus is involved in dopamine signaling. Under pathological conditions, α-synuclein can undergo conformational changes leading to protein aggregation and deposition.α-Synuclein shares structural homology with the 14-3-3 family of chaperon proteins and its expression is upregulated during periods of accelerated neuronal activity and plasticity thus suggesting it might be functioning as a molecular chaperon capable of binding to other intercellular proteins.α-Synuclein has been implicated in the pathophysiology of Parkinson's disease (PD) and Lewy body dementia (DLB). Mutations A30P and A3T are associated with PD while mutation E46K has been linked to DLB. These mutated α-synucleins form fibrils more rapidly than the wild-type protein in aqueous solution1. α-Synucleins are the major components of Lewy bodies and Lewy neurites in both diseases2.

ID gène: 29219

NCBI Accession: NM_000345

UniProt: P37377

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity