VAMP2 anticorps (Cytosolic, N-Term)

N° du produit ABIN7043878

Cet anticorps Lapin Polyclonal détecte spécifiquement VAMP2 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour VAMP2 anticorps (Cytosolic, N-Term) (ABIN7043878)

Antigène: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VAMP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-VAMP2 anticorps

Épitope: AA 2-27, Cytosolic, N-Term

Fonction: A Rabbit Polyclonal Antibody to VAMP-2

Réactivité croisée: Souris, Rat

Homologie: Human,bovine,Macaca mulata - 25,26 amino acid residues identical

Attributs du produit: Anti-v Antibody is directed against the intracellular N-terminal domain of rat V. Anti-V Antibody (ABIN7043878, ABIN7044653 and ABIN7044654) can be used in western blot and immunohistochemical applications, and has been designed to recognize V from rat, mouse and human samples.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogène: GST fusion protein with the sequence SATAATVPPAAPAGEGGPPAPPPNLT, corresponding to amino acid residues 2-27 of rat VAMP2

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7566008)

Blocking Peptide: (ABIN7566008)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur VAMP2

Antigène: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Autre désignation: VAMP2

Sujet:

Synonyms: Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2

Description: VAMP-2 (also known as synaptobrevin-2) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionary conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.VAMP-2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located on the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.VAMP-2 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as the vesicle membrane protein v-SNARE, which together with the plama membrane t-SNARE protein Syntaxin 1 and the membrane-associated SNAP-25 (synaptosome-associated protein 25 kDa), forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.VAMP-2 is the target of the tetanus neurotoxin (TeNT) and of several botulinum neurotoxin (BoNT) types: type B, D, F, and G. The neurotoxins cause specific proteolytic degradation of the VAMP-2 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.

ID gène: 24803

NCBI Accession: NM_001330125

UniProt: P63045

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport