Recombinant Rhodopsin anticorps (C-Term)

N° du produit ABIN7072289

L’anticorps anti-Rhodopsin Chimeric Souris est utilisé pour la détection de Rhodopsin dans des échantillons de Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio) et Amphibien. Il a été validé pour WB, IHC, ELISA, IP, IF et Lbl.

Aperçu rapide pour Recombinant Rhodopsin anticorps (C-Term) (ABIN7072289)

Antigène: Rhodopsin (RHO)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Amphibien

Hôte: Souris

Clonalité: Chimeric

Conjugué: Cet anticorp Rhodopsin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Labeling (Lbl)

Clone: Rho 1D4

Détail du produit anti-Rhodopsin anticorps

Épitope: C-Term

Fonction: Anti-Rhodopsin [Rho 1D4], Mouse IgG1, kappa

Specificité:

The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Application Notes original Clone: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

Réactivité croisée: Amphibien, Boeuf (Vache), Humain, Rat, Poisson zèbre (Danio rerio)

Attributs du produit: This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: Bleached bovine rod outer segment (ROS).

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Rhodopsin

Antigène: Rhodopsin (RHO)

Autre désignation: Rhodopsin

Classe de substances: Chemical

Sujet: Rho-tag, Visual Purple

UniProt: P08100

Pathways: Signalisation WNT, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction